What is MS2 coat protein?

What is MS2 coat protein?

The bacteriophage MS2 coat protein specifically binds to a small RNA hairpin in its genomic RNA (1–4). The complex represses translation of the early replicase gene and identifies the RNA for future packaging into virus particles by forming an initiation complex.

What is MS2 RNA?

Bacteriophage MS2 (Emesvirus zinderi), commonly called MS2, is an icosahedral, positive-sense single-stranded RNA virus that infects the bacterium Escherichia coli and other members of the Enterobacteriaceae.

What does MS2 detected mean?

MS2 Internal Control: MS2 in a patient result indicates that PCR amplification occurred in the well. The presence of MS2 and no detectable SARS-CoV-2 during the analysis indicates that proper RNA extraction and amplification occurred, however, no SARS-CoV-2 is present.

How does MS2 system work?

MS2 tagging and similar aptamer-based methods involve cloning unique sequences (MS2 hairpins, RNA aptamer sequences, etc.) into the 3′UTR of the transcript of interest; this sequence is then recognized and bound by a fluorescently tagged protein or other molecule using live or fixed cells.

Why is a genome needed for RNA Seq analysis?

Not limited to genomic sequences – unlike hybridization-based approaches, which may require species-specific probes, RNA-seq can detect transcripts from organisms with previously undetermined genomic sequences. This makes it fundamentally superior for the detection of novel transcripts, SNPs or other alterations.

Is MS2 an antibody?

The bacteriophage MS2 is a small, male-specific RNA bacteriophage that infects E. coli and other members of the Enterobacteriaceae family….Key Specifications Table.

Why is MS2 used as a control?

MS2 Bacteriophage is used as a standard to study molecular biology processes. MS2 can be used as an Internal Control in Viral Reverse Transcription-PCR Assays to verify the efficacy of the sample preparation and absence of inhibitors in the PCR reaction.

What is MS2 used for?

The F+-specific coliphage MS2 has been widely used as a surrogate for human enteric viruses in many studies on virus transport, disinfection, and fate (1, 5, 17, 20, 21). Here we present an MS2 NAT for different real-time RT-PCR approaches, which was used to monitor RT-PCRs for the detection of human RNA viruses.

What is the purpose of RNA-Seq?

RNA-seq (RNA-sequencing) is a technique that can examine the quantity and sequences of RNA in a sample using next-generation sequencing (NGS). It analyzes the transcriptome, indicating which of the genes encoded in our DNA are turned on or off and to what extent.

What can we do with RNA-Seq data?

RNA‐seq can be a powerful tool to measure gene expression, detect novel transcripts, characterize transcript isoforms, and identify sequence polymorphisms.

What does the word bacteriophage mean?

bacteria eater
A bacteriophage is a type of virus that infects bacteria. In fact, the word “bacteriophage” literally means “bacteria eater,” because bacteriophages destroy their host cells.

What is MS2 internal control?

MS2 Bacteriophage can be used as an internal control in nucleic acid-based amplification assays to monitor reverse-transcription and PCR inhibition. Controls should be run using the same protocols as those used to amplify extracted clinical specimens.