Does Gal4 bind to DNA?

Does Gal4 bind to DNA?

Gal4 is an 881-amino-acid protein with a Zn–Cys binuclear cluster-type DNA-binding domain, a linker domain, a dimerization domain and two acidic activation domains (ARI and ARII; Lohr et al, 1995).

What is Gal4 gene?

The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes. This protein represents a large fungal family of transcription factors, Gal4 family, which includes over 50 members in the yeast Saccharomyces cerevisiae e.g. Oaf1, Pip2, Pdr1, Pdr3, Leu3.

What is the molecular function of the Gal4 protein?

How does the Gal4 system work?

Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. You can quickly mate your UAS-YFG line with a variety of Gal4 lines and study gene expression in many different tissue types!

Is Gal4 an activator?

Yeast Gal4, one of the first known acidic activators, is responsible for the regulation of genes involved in galactose catabolism (41). In the absence of galactose, Gal4 is inhibited by the repressor Gal80.

What is the effect of Gal80 binding on Gal4 function?

The Saccharomyces cerevisiae Gal80 protein has two binding partners: Gal4 and Gal3. In the absence of galactose, Gal80 binds to and inhibits the transcriptional activation domain (AD) of the GAL gene activator, Gal4, preventing GAL gene expression.

How does Gal80 inhibit Gal4?

In the absence of galactose, the Gal80 protein binds to a small peptide (amino acids [aa] 855 to 870) within the Gal4AD and thereby prevents Gal4-mediated promoter activation (10, 11). Galactose converts Gal3 to a form that readily binds to Gal80 (21).

What functional domains does the yeast Gal4 protein possess?

These observations imply that GAL4 protein consists of several domains, including ones for nuclear localization, interaction with the negative regulatory protein, and, possibly, separable transcriptional activation domains for the structural genes.

Is GAL4 an activator?

Where is GAL4 found?

For study in Drosophila, the GAL4 gene is placed under the control of a native gene promoter, or driver gene, while the UAS controls expression of a target gene. GAL4 is then only expressed in cells where the driver gene is usually active.

What chromosome is GAL4 on?

Where is GAL4 found in the cell?

gene promoter
For study in Drosophila, the GAL4 gene is placed under the control of a native gene promoter, or driver gene, while the UAS controls expression of a target gene. GAL4 is then only expressed in cells where the driver gene is usually active.