How much insulin is in FBS?
I know that insulin is about 40 pM in 5% FBS, but what about the other hormones? And does anyone have a protocol how to remove insulin from FBS? (affinity purification?) Charcoal-stripping is a not option for me since it removes also other hormones and compounds.
What is the composition of FBS?
There are 1000+ components found in FBS, including proteins, electrolytes, lipids, carbohydrates, hormones, enzymes, and other undefined constituents, which are necessary in many culture conditions to support cell growth.
What is the protein concentration in FBS?
The medium we used in this study contains 4.1 mg/ml protein as detected by BCA method. The major component of FBS is serum albumin which constitutes 60 to 67% of total proteins.
Is there insulin in fetal bovine serum?
A total of 143 proteins were identified in FBS, among which 14 proteins including insulin-like growth factor 2 (IGF-2) and IGF binding protein (IGFBP)-2 and -6 were reduced in CS-FBS.
What is the normal range of fasting insulin?
|Insulin Level||Insulin Level (SI Units*)|
|Fasting||< 25 mIU/L||< 174 pmol/L|
|30 minutes after glucose administration||30-230 mIU/L||208-1597 pmol/L|
|1 hour after glucose administration||18-276 mIU/L||125-1917 pmol/L|
|2 hour after glucose administration||16-166 mIU/L||111-1153 pmol/L|
How is fasting insulin measured?
(2001) Score for measuring the Insulin Sensitivity Index: The ISI is calculated for fat-free body mass by dividing the glucose disposal rate (M – mg/kg/min) by the average plasma insulin concentration over the final 60 minutes of the 120 minute test. An ISI of 6.3 M/mU/l defined individuals with insulin resistance.
Does FBS contain glucose?
Thermo fisher FBS contains less than 50 mg/L of glucose and using the same should not affect your result.
What growth factors are in FBS?
FYI, according to Thermo’s data, 111ng/ml IGF, 12.6ng/ml TGF-beta, 37.3ng/ml FGF-2 is present in FBS.
What is the difference between BSA and FBS?
Is Bovine Serum Albumin (BSA) and Fetal Bovine Serum (FBS) the Same Thing? Nope. It’s not the same. Fetal Bovine Serum is a commonly used serum supplement for eukaryotic cell culture.
Does FBS contain albumin?
Bovine serum albumin is the major component of FBS. Growth factors in FBS are essential for the maintenance and growth of cultured cells [1, 2]. FBS also contains a variety of small molecules like amino acids, sugars, lipids, and hormones.
Does FBS contain estrogen?
The FBS is widely used in the supplementation of culture media because it contains a large amount of biological components, such as fatty acids, growth factors, aminoacids, vitamins, and hormones, including estrogen (Hemeda et al. 2014; Tekkatte et al.
How do you make charcoal stripped serum?
Preparation of charcoal-stripped serum Use a volume of the dextran-coated charcoal (DCC) from step 1 equivalent to that of the serum which is to be stripped. Centrifuge it (500 x g for 10 min) to pellet the charcoal. Decant the supernatant and replace it with the same volume of FBS.
What is the optimal concentration of insulin in cell culture media?
Insulin is typically added to cell culture media at concentrations ranging from 1 to 10 milligrams per liter of medium, which is roughly 100-fold the physiological concentration. At these concentrations, Insulin can mediate a broader range of beneficial effects by interacting with both the insulin and IGF-1…
Does anyone know the exact concentration of FBS in serum?
I do not think you would find information about exact concentrations of GF in serum. Vendors usually test FBS on toxicity, sterility (inc. viruses), growth-supporting potential, and probably on total composition, but unlikely to do measurements of exact concentrations of GF.
What is the use of FBS in cell culture?
FBS is used in cell culture of eukaryotic cells, and has a wide range of applications. For example, it’s used in the research, manufacture, and control of human and veterinary vaccines and of biotech drugs. Often human cell lines and primary cells are cultured with FBS.
How much TGF-beta and IGF in FBS?
FYI, according to Thermo’s data, 111ng/ml IGF, 12.6ng/ml TGF-beta, 37.3ng/ml FGF-2 is present in FBS. But as said above, there are big variations between batchs.