What do ade1 and ade2 mutations tell us about oxidative stress?
Use of ade1 and ade2 mutations for development of a versatile red/white colour assay of amyloid-induced oxidative stress in saccharomyces cerevisiae Mutations in adenine biosynthesis pathway genes ADE1 and ADE2 have been conventionally used to score for prion [PSI+ ] in yeast.
How is the ade2-101 mutation overcome by a suppressor?
In this system the ade2-101 mutation is overcome by a suppressor of ochre 92 mutations. Due to the instability of the plasmid bearing the suppressor gene, the colonies are sectored. If, however, an essential gene is also dependent on the presence of the ochre suppressor, no segregation of the plasmid and thus no sectoring can occur.
What does ade2 stand for in genetics?
INTRODUCTION Mutants in the S. cerevisiae gene ADE2 (encoding phosphoribosylamino-imidazole-carboxylase, EC 4.1.1.21) have widely been used because they accumulate a red pigment (Ephrussi et al., 1949; Reaume and Tatum, 1949; Roman, 1956).
How are acquired mutations not passed down?
Acquired mutations are not passed down if they occur in the somatic cells, meaning body cells other than sperm cells and egg cells. Some acquired mutations occur spontaneously and randomly in genes. Other mutations are caused by environmental factors, such as exposure to certain chemicals or radiation.
What is the Saccharomyces cerevisiae ade1 gene?
Myasnikov AN, et al. (1991) The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control. Gene 109 (1):143-7 PMID: 1756975 Jones EW and Fink GR (1982) “Regulation of amino acid and nucleotide biosynthesis in yeast.”
How do I view all alleles in yeastmine?
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click “SGD search” to view all alleles in search results. Click “YeastMine” to view all alleles in YeastMine. Show all Show fewer
How do I view down-regulated and up-regulated gene expression data?
The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click “Expression Details” to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.