What is 1KB plus DNA ladder?

1KB Plus DNA Ladder is a premixed, ready-to-load molecular weight marker containing eight linear double-stranded DNA fragments. The DNA Ladder is suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA Ladder contains 300bp -10.0 kb DNA fragments.

How do you prepare 1 kb of DNA ladder?

If you want to prepare a working stock of the 1 kb ladder with loading dye, measure amount of ladder in the tube and add the loading dye accordingly. Use water to dilute your loading dye, in the ratio of 1 part ladder, 1 part buffer, and 4 parts ddH20 (if your loading buffer is 6X).

How much ladder do I add to gel?

For a standard electrophoresis system, we recommend loading 0.5 µg (20 µl) of the Fast DNA Ladder on the agarose gel. For a fast electrophoresis system (5 to 30 minutes separation), follow the system’s manufacturer recommendations: 5 to 20 µl load.

What is the highest DNA band size in 1 kb DNA ladder?

Description: The 1 Kb Plus DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 100 bp to 12 kb. The ladder contains a total of twenty bands: twelve bands ranging in size from 1000 bp to 12,000 bp in 1000-bp increments and eight bands ranging in size from 100 to 1650 bp.

How do you dilute 100 bp DNA ladder?

This ladder is preferred by our lab because of its broad range (100 bp to 10,000 bp) and even intervals. Its stock concentration is 1 µg/µl of DNA. The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder.

What does bp mean in DNA ladder?

DNA molecular weight (mw) standard controls of nucleic acids, also known as DNA ladders, are widely used in molecular biology studies to determine the mw or the base pair (bp) length of nucleic acids. The DNA ladder is also used to quantitatively analyze target DNA fragments.

What is a DNA ladder?

DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. Once being cloned, the tandem repeat was rapidly amplified by PCR and partially digested by restriction enzymes to produce a ladder containing multimers of the repeated DNA fragments.

What is generuler 1 KB plus DNA ladder?

Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. Highlights of 1 kb Plus DNA Ladder

What is Invitrogen 1 KB plus DNA ladder?

Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.

How many KB plus DNA ladder can be visualized by ethidium bromide?

1 kb Plus DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane. Suggested Load: 5 to 10 µl/lane. The following reagents are supplied with this product:

What is generuler 1 KB plus?