What is 3xFLAG?
The 3xFLAG® system is an improvement upon the original system by fusing three tandem FLAG® epitopes for a total of 22 amino acids (Figure 1). Detection of fusion proteins containing 3xFLAG® is enhanced up to 200 times more than any other system.
Why 3xFLAG tag?
The increased length of the 3x Flag-tag increases the affinity of the anti-Flag antibody/affinity reagents. 3x Flag-tag is often used in tandem purification and protein purification. As mentioned above, 3x-Flag-tag is longer than 1x Flag-tag and has more charged amino acids.
What is Flag plasmid?
FLAG® Tag Expression Vector Maps and 3xFLAG® Peptides Bacterial and mammalian expression vectors, or expression constructs, are circularized DNA transfection plasmids that are commonly used to overexpress recombinant proteins of interest.
Is DDK a flag tag?
DYKDDDDK Tag (FLAG® tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology.
What is the size of myc tag?
5 x 1 mg
Description
| Target: | c-Myc |
|---|---|
| Product Type: | Peptide |
| Size: | 5 x 1 mg |
| Research Area / Disease: | Epitope Tag |
| Gene ID Human: | 4609 |
Is DDK a FLAG-tag?
What is the molecular weight of flag?
1012.98
16. Flag- vs. V5-tag
| Flag-tag | V5-tag | |
|---|---|---|
| 3D structure | ||
| Amino acid sequence | DYKDDDDK | GKPIPNPLLGLDST |
| Number of amino acids | 8 | 14 |
| Molecular weight (in Da) | 1012.98 | 1421.66 |
How big is a flag tag?
about 1 kDa
FLAG is a short, 8 amino acid sequence DYKDDDDK about 1 kDa in size (D = aspartic acid, Y = tyrosine, K = lysine). Due to its small size, some researchers may choose to leave the tag intact, but cleavage can be performed with enterokinase.
What is the 3xFLAG® system?
The 3xFLAG ® system is an improvement upon the original system by fusing 3 tandem FLAG ® epitopes (22 amino acids). Detection of fusion proteins containing 3xFLAG ® is enhanced up to 200 times more than any other system. Like the original FLAG ® tag, 3xFLAG ® is hydrophilic, contains an Ek cleavage site, and is relatively small.
What are the binding sites of the flag® and 3xFLAG® sequences?
The FLAG ® and 3xFLAG ® sequences include the binding sites for several highly specific ANTI-FLAG ® monoclonal (M1, M2, M5) and polyclonal antibodies and conjugates, each with different recognition and binding characteristics. ANTI-FLAG ® antibodies exhibit little or no cross-reactivity in most mammalian and bacterial cell lysates.
What is the Anti-Flag ® expression system?
These small hydrophilic tags facilitate superior detection and purification of recombinant fusion proteins when using our highly specific and sensitive ANTI-FLAG ® antibodies. The FLAG ® expression system utilizes a short, hydrophilic 8-amino acid peptide that is fused to the recombinant protein of interest.
Can enterokinase remove Flag and 3xFLAG tags?
Therefore, the risk of altering protein function, blocking other epitopes or decreasing solubility is minimized. Removal of N-terminal FLAG ® (8 amino acids) and 3xFLAG ® (22 amino acids) tags is possible using enterokinase, which cleaves following the Asp-Asp-Asp-Asp-Lys amino acid sequence at the c-terminal end of the tags.