What is A2780 cell line?
Cell Line Description The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (Sigma catalogue no.
What is mcf7 cell line?
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers.
Where do cell line culture originate from?
Cell lines are cultures of animal cells that can be propagated repeatedly and sometimes indefinitely. They arise from primary cell cultures. Primary cultures are initiated directly from the cells, tissues, or organs of animals and are typically used in experiments within a few days.
What is OVCAR3?
NIH:OVCAR-3 [OVCAR3] are epithelial cells that were isolated in 1982 from the malignant ascites of a patient with progressive adenocarcinoma of the ovary. Use these cells to study drug resistance in ovarian cancer.
Is MCF-7 invasive?
Though MCF7 is categorized as poorly invasive, its migration in the 2D surface is found to be comparable to the highly invasive MDA MB 231 in the current study.
Do MCF-7 cells overexpress HER2?
MCF-7 breast cancer cells stably overexpressing ERbeta1 exhibited elevated >5-fold HER2 mRNA levels and elevated >3-fold HER2 protein levels even in the absence of estradiol.
How are cell lines derived?
Primary cell lines are derived directly from excised tissue and cultures either as an explant culture or following dissociation into a single cell suspension by enzyme digestion. Such cultures are initially heterogeneous but later become dominated by fibroblasts.
What is the difference between a primary culture and a cell line?
Primary cell culture is the culture of cells directly isolated from parental tissue of interest; whereas cell line is the culture of cells originated from a primary cell culture, which is generally used to expand cell population and prolong life span.
How do I choose the right cell line for my research?
The Do’s and Dont’s of Choosing a Cell Line
- Don’t Choose Simply Because It Is Used in the Literature.
- Do Consider If It Fits Your Biological Model.
- Do Consider the Experiments You Want to Perform.
- Table 1: Features of commonly used cell lines.
- Don’t Assume That All Cell Lines Have the Same Culturing Requirements.
Does MCF-7 have HER2?
MCF-7 and HER2 MCF-7 cells do not have amplification of the HER2 (ErbB2) oncogene. But given the paucity of models for the study of HER2 and the potential cross-talk between ER and HER2, Osborne and Benz generated MCF-7 cells that overexpress HER2 ( 23 ).
What is the A2780 cell line?
Tumour line A2780 cell line used to investigate cisplatin resistance in ovarian cancer. The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient of ovarian endometroid adenocarcinoma. Cells grow as a monolayer and in suspension in spinner cultures.
What is the culture medium for A2780 C-cell line?
Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (ECACC catalogue no. 93112517) and the adriamycin resistant cell line A2780 ADR (ECACC catalogue no. 93112520). Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
What is the difference between A2780 and A2780 ADR?
Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (catalogue no. 152708) and the adriamycin resistant cell line A2780 ADR (catalogue no. 152707).
What is ECACC?
ECACC supplies authenticated and quality controlled cell lines, nucleic acids and induced Pluripotent Stem Cells (iPSCs). ECACC was established in 1985 as a cell culture collection to service the research community and provide an International Depository Authority recognised patent depository for Europe.