What is BV605?
BV605. The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm.
How bright is BV605?
On a brightness scale of 1-5 with 5 being the brightest, we would give this a 5, although not as bright as BV421™. BV605™ has very little cross-beam compensation requirements with the 488nm laser. However, there may be some spillover into the PE-TR detector on 561/532nm laser-equipped instruments.
Is BV421 same as Pacific Blue?
For most cell surface staining, BV421™ is significantly brighter than most equivalent fluorophores including Pacific Blue™, BD Horizon™ V450, eFluor® 450, and Alexa Fluor® 405. It has an extinction coefficient of 2,500,000 M-1cm-1 at 405 nm, and an aqueous solution quantum yield of 65 ± 5%.
What is the brightest fluorophore?
It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.
What is BV711?
405 nm 710/50 BD Horizon™ Brilliant Violet™ 711 (BV711) (Exmax 407 nm/Emmax 711 nm) is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em Max at 711 nm. This dye offers a very bright choice for the violet laser.
Is Pacific Blue a tandem dye?
Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm. Pacific Blue is a trademark of ThermoFisher. This dye comes from the ‘Pacific’ dye product line manufactured by Invitrogen (ThermoFisher) that includes Pacific Blue, Pacific Green, and Pacific Orange.
Is BV605 a tandem dye?
405 nm 610/20 BD Horizon Brilliant™ Violet 605 (BV605) (Exmax 407 nm/Emmax 602 nm) is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 602 nm. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates.
Is APC brighter than PE?
Compared to PE, APC is not as”bright”. This is due in part to its lower extinction coefficient and quantum yield. It is also because APC is red-shifted compared to PE. Thus, the light source used to maximally excite APC will contain less energy than the one used to maximally excite PE.
Can you use PE and FITC together?
Relative contribution. In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. In those cases the relative contribution of each fluorophore to the signal in a given detector must be determined (Figure 11).
What color is BV510?
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm.
What is an Alexa Fluor conjugate?
Protein conjugates made with Alexa Fluor dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. See all Alexa Fluor Primary Antibodies.
Which is brighter PE or APC?
Compared to PE, APC is not as”bright”. Compared to other red fluorophores such as Cy5, however, APC is still significantly brighter and an excellent choice for use in flow cytometry.
How does the bv605 work?
BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover.
What is BD horizon bv605 antibody?
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set.
What is tandem fluorochrome bv605?
This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter).
What is BD horizon brilliant violet 605?
The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm.