What is in RPMI media?
One liter of RPMI 1640 contains: Glucose (2 g) pH indicator (phenol red, 5 mg) Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
What is the difference between RPMI and DMEM?
RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.
Does RPMI contain FBS?
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
Why is RPMI used?
RPMI is used when laboratory testing requires fresh, unfixed specimens such as flow cytometry as well as some FISH, molecular and cytogenetics tests. 2 ) Why is it pink/red? It has a phenol red pH indicator that will turn from pink to yellow indicating contamination or exposure to air.
Can I use DMEM instead of RPMI?
On a very basic level in our laboratory you would use DMEM for an adherent cell line and you would use RPMI for suspension cell lines. For example some cell lines grow and flourish better in the presence of HEPEs. Also you need to think about your down the line uses for the cells.
What is the difference between IMDM and RPMI?
One major difference in the formulations of RPMI versus IMDM of potential relevance for PMA/ionomycin stimulation is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM.
How can I get 10 FBS?
get 200 ml MQ H2O, weigh 4 g Gelatin (cell culture grade) and add to H2O, gently shake bottle, put on a tray containing some water, loose the cap of the bottle, autoclave with liquid cycle (30min). After take out, be sure to shake the bottle until gelatin desolve uniformly in liquid, keep in refrigerator for later use.
Why is RPMI used for suspension cells?
Calcium concentration is different in each media, the once in RPMI is the lowest and this fact is favourable for many cell types, as are the cells in suspension, and for example, pancreatic beta cells.
Is GlutaMAX the same as L-glutamine?
Gibco GlutaMAX supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture media. GlutaMAX supplement is a dipeptide, L-alanine-L-glutamine, which is more stable in aqueous solutions and does not spontaneously degrade.
What is DMEM used for in cell culture?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What is the difference between Imdm and DMEM?
IMDM is a modification of DMEM containing selenium, and has additional amino acids, vitamins and inorganic salts as compared to DMEM. It has potassium nitrate instead of ferric nitrate and also contains HEPES and sodium pyruvate.
Why do we use 10% FBS?
As I know we always use media with 10% FBS. FBS provide media for growth of cells. FBS consist of some growth supplement for cells and without FBS cells are no able to growth normally. If you check the ATCC (atcc.org) website you found that cells need media (RPMI, DMEM, MEM,…) with 10% FBS.