What is May Grunwald Giemsa stain used for?
May Grunwald-Giemsa (MGG) Stain is used for staining of blood, bone marrow smears and clinical cytological specimens. May Grunwald-Giemsa (MGG) staining method is used for morphological inspection and differential counting of blood cells.
What is the principle of Grunwald Giemsa stain?
PRINCIPLE: The “neutral” dyes combining the basic dye methylene blue and the acid dye eosin, give a wide color range when staining. The pH of the staining solution is critical and ideally should be adjusted for different fixatives.
How do you make may Grunwald stain?
Combine 30 ml of May-Gruenwald solution with 150 ml of distilled or demineralized water and with 20 ml of buffer solution. Add 10mL of the Giemsa solution to 190 ml of pH 6.8 buffer solution, stir well and let it sit for 10 min. Filtrate if necessary.
What is Giemsa stain method?
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
What are the components of the May-Grünwald stain?
May-Grünwald is alcohol based and contains May-Grünwald’s eosin methylene blue and methanol (>85%). Giemsa is alcohol based and contains Giemsa’s azure eosin methylene blue, methanol (>50%) and glycerol.
How do you stain a blood smear with Giemsa?
Method
- Deparaffinize and rehydrate through graded alcohols to water.
- Rinse in pH 6.8 buffered distilled water.
- Stain in working Giemsa, overnight.
- Rinse in distilled water.
- Rinse in 0.5% aqueous acetic acid until section is pink.
- Wash in tap water.
- Blot until almost dry.
What is the principle of Leishman stain?
Leishman stain contain methylene blue dye, a basic dye, which gives colour to an acidic component and eosin dye, an acidic dye, which gives colour to a basic component. These dyes differentiate the different component of blood.
What is the function of glycerol in Giemsa stain?
In 1904 Giemsa published an essay on the staining procedure for flagellates, blood cells, and bacteria. Giemsa improved the Romanowsky stain (Eosin Y and Methylene Blue) by stabilizing this dye solution with glycerol. This allowed for reproducible staining of cells for microscopy purposes.
Which fixative is used in MGG stain?
May Grunwald’s Blood Stains are basic and acidic dyes which induce multiple colours when applied to cells. Methanol acts as a fixative and also as a solvent.
How does Wright Giemsa stain work?
Wright Giemsa Stain is a modified Romanowsky Stain technique, which is used to stain the smears of blood and marrow. The Staining of cells involves physical adsorption and chemical affinity which allows the stain to penetrate and remain within cells.
Is Giemsa stain acidic or basic?
Principle of Giemsa Stain Azure and eosin are acidic dye that variably stains the basic components of the cells like the cytoplasm, granules etc. Methylene blue acts as the basic dye, which stains the acidic components, especially the nucleus of the cell. Methanol act as a fixative as well as the cellular stain.
What type of stain is Giemsa?
nucleic acid stain
Giemsa stain (/ˈɡiːmzə/), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites.
What is May Grunwald Giemsa stain?
Newcomer Supply May-Grunwald Giemsa (MGG) Stain procedure for paraffin sections, is used for differential staining of hematopoietic tissue as well as demonstration of some microorganisms. May-Grunwald refers to a combined stain of eosin and methylene blue; the Jenner Stock Stain.
How do you use Giemsa stain?
Flood the slide with 5% Giemsa stain solution for 20-30 minutes. Add a thick smear of blood and air dry for 1 hour on a staining rack. Dip the thick blood smear into diluted Giemsa stain (prepared by taking 1ml of the stock solution and adding to 49ml of phosphate buffer or distilled water, but the results may vary differently).
What is Maymay-Grunwald stain made of?
May-Grunwald refers to a combined stain of eosin and methylene blue; the Jenner Stock Stain. Giemsa refers to a mixture of methylene blue, eosin and Azure B; the Giemsa Stock Stain, Wolbach. The use of Jenner Stock Stain and Giemsa Stock Stain, Wolbach, provides intense colorization results in the MGG Stain.
What is the difference between Maymay-Grunwald and Giemsa?
May-Grunwald refers to a combined stain of eosin and methylene blue; the Jenner Stock Stain. Giemsa refers to a mixture of methylene blue, eosin and Azure B; the Giemsa Stock Stain, Wolbach.